IVT RNA Synthesis Services

Why GenScript

Accelerate Discovery

Industry-leading 2 weeks turnaround time from Gene-to-mRNA for global customers

• Integrated capabilities from sequence design to RNA synthesis and LNP formulation in one solution
• Delivered >10000 quality mRNA orders globally with 93% on-time delivery 

Cultivate Collaboration

Proprietary & patented GenScript Cap™ technologies open to licensing

Patented technologies covers:

• Co-transcriptional capping Method
• Selection of UTRs for tissue specific targeting
• Optimized PolyA vector

Case Study

• New Cap Analogue 2’-O-Ethyl-capped RNA Enhance Half Life In Vivo
  

  

  Novel 2’-O-Ethyl-capped RNA showed higher expression than natural Cap1 RNA, and slower clearance rate in vivo.

FAQs

• What is the length limit of GenScript's mRNA production?

  We can make mRNA from 100bp to 20kb in length. For oligo shorter than 100bp, our oligo synthesis team can take it under chemical syntehsis.

• What’s the difference between RUO and preclinical grade?

  For RUO grade mRNA, we use silica membrane or LiCl precipitation method for purification. It is suitable for in vitro and invivo proof of concept studies.

  For preclinical grade, we use HPLC based Oligo dT purification for improving RNA purity. Preclinical grade can be considered for long mRNA constructs >5kb or when high RNA purity (less fragment RNA ) is required for the project.

• Can GenScript provide GMP mRNA ?

  We can provide cGMP mRNA through our sister company ProBio. Please contact our technical support team.

• Do you guarantee a specific mRNA yield/amount?

  Yes. We guarantee specific amounts of mRNA be delivered.

• What concentration can you ship my mRNA at?

  We ship mRNA at concentration of 0.5 ~2mg/mL by default. We can adjust concentration to a specific value <4mg /mL per customer's request at extra cost.

• What buffer options does GenScript provide for mRNA ?

  We offer 3 options of buffer: 1mM sodium citrate pH 6.5, TE pH7, and RNase-Free H2O. 1mM sodium citrate is our default option for better mRNA stability.

• How should I store my mRNA and long RNA?

  mRNA should be stable for at least 1 year if stored properly at -80°C upon receival and avoiding repeated freezing and thawing cycles, however mRNA stability varies depending on the length of mRNA. We have tested mRNA with stable results up to 3 freeze/thaw cycles. We recommend storing at -80°C for long time storage and -20°C for short term storage.

• How does GenScript purify mRNA?

  For RUO grade mRNA, we use silica membrane or LiCl precipitation method for purification.

  For preclinical grade, we use HPLC-based Oligo dT purification for improving RNA purity.

  In addition, we offer optional dephosphorylation to further reduce triphosphate RNA species in the final product to reduce its immunogenicity, and optional dsRNA removal HPLC purification to provide higher quality mRNA if low dsRNA residue is required.

• What method does GenScript use for dsRNA removal?

  Cellulose based HPLC method.

• How to define the purity for mRNA?

  For size-based purity, we run the mRNA sample on bioanalyzer, then calculate the ratio of the main peak (target size +-15%) over the total AUC.

• What’s the function of dephosphorylation?

  We recommend customers dephosphorylation as reducing the triphosphate-RNA species in mRNA by dephosphorylation could reduce its immunogenicity, thus making the transfected cells healthier and can express the target protein better.

• What’s the function of modification of mRNA?

  Modifications help stabilize mRNA and reduce its immunogenicity.

• What modified bases does GenScript offer?

  We can substitute uridine bases with either 5-moU, N1-me-Ψ, Ψ, 5’-iodourdine, or Cy5-UTP.

  We can substitute cytidine bases with 5-me-C, 5’-iodocytidine, or thiol-CTP.

  We can substitute customizable percentage substitutions upon request. These will be not site specific modifications.

• What’s the function of capping?

  Capping can stablize RNA from 5'end and recruit ribosome. Cap1 and Cap 2 have a higher impact on mRNA stability compared to Cap 0 regarding protecting the mRNA from exonuclease degradation. It is common to use Cap1 for mammalian cells and Cap 0 for plant cells. Cap2 is a novel solution provided by GenScript that may work better than Cap1 in mammailan cells especiailly in immune-stressed conditions.

• What 5’ cap does GenScript offer?

  Cap1 via co-transcriptional capping is our default recommendation. We can also offer Cap0 and Cap1 enzymatically for an additional cost, and Cap2 RNA through co-transcriptional capping technology.

• How is the capping efficiency of GenScript's mRNA platform?

  GenScript's proprietary capping technology can achieve >97% capping efficiency.

• Does GenScript provide Cap Efficiency analysis as stand-alone service?

  Yes, we offer mRNA QC service including capping efficiency assay using enzyme digestion /LC-MS method. Please contact our technical support team for details.

• What template does GenScript use for mRNA manufacture? How is that made?

  We use linearized DNA plasmid as a template for manufacturing your mRNA. The gene can be designed by our team using GenScript’s proprietary vectors and tools or we can use one of your designs. Once the design has been agreed to, we handle the gene and mRNA manufacture and provide you with mRNA as part of the fee-for-service agreement.

• Can I use my own UTRs for GenScript to produce mRNA?

  Yes, it is possible, please contact our technical support team.

• What is the requirement for T7 promoter?

  For making cap1 mRNA using co-transcriptional capping technology, either AGG or GGG following T7 promoter seq is required. For self-amplifying RNA usually ATG after T7 promoter.

• Does GenScript provide mRNA codon optimization?

  Yes, we can do codon optimization. Please contact our technical support team.

• Can you use plasmids I have already made with GenScript in previous gene synthesis orders for mRNA production?

  Yes. we can subclone your ORF sequences into our proprietary vector which contains T7 promoter, UTR and 100A for mRNA production.

• For the poly A length assay, what enzyme does GenScript use?

  We use RNaseT1.

• Can GenScript perform quality testing on customer-provided mRNA?

  Yes, please refer to https://www.genscript.com/mrna-qc-services.html.

• How much mRNA is needed to transfect cell cultures in 24-well plates?

  Approximately 0.5ug to 1 ug of mRNA is usually used for transfecting each well in 24 well plate.

Testimonials

Dr. Sean Whelan

Washington University School of Medicine

Contracting with Genscript for the production of mRNA-LNPs has advanced my laboratory's research into viral vaccines by allowing us to rapidly produce and test multiple mRNA vaccine constructs for several different viruses. We appreciate Genscript's easy ordering processing, and diverse options for mRNA format and LNP encapsulation strategy.

Dr. Mahdi Zeraati

University of Sydney

We have used their IVT mRNA synthesis, FLASH gene synthesis, and TurboCHO antibody production platforms across several projects, and each has consistently met our expectations. Notably, GenScript successfully synthesized a challenging, repeat-rich gene that other providers declined to undertake, demonstrating their technical capability and commitment to supporting complex research needs.

Chun-YuChen

Seattle Children's Hospital

I purchased mRNA from different companies and found that GenScript's mRNA showed higher expression (almost 2 fold) and much more cost effective!!

Dr. Sang-Moo Kang

Institute for Biomedical Sciences at Georgia State University

GenScript mRNA has helped us make a rapid progress in developing novel vaccine projects. GenScript's service is faithful and timely provided, saving me lots of time to focus on research. This is why I have been using GenScript services for over 20 years.

Zack Ho

R&D Manager at Lion TCR Pte Ltd

We have utilized GenScript's gene and mRNA synthesis service for our research projects, and the outcomes have been exceptional. The product quality, timely delivery, and technical support have consistently surpassed our expectations.

Resources

mRNA Case Study Report

Case studies showing high expression efficiency of GenScript’s mRNA for expressing SB transposase, GFP, Luciferase, eSpCas9, and more.

Free Download

Circular RNA Case Study Report

Circular RNA introduction, GenScript offering details, and case studies showing increased stability, prolonged expression, and lower immunogenicity compared to linear IVT mRNA.

Free Download

LNP Case Study Report

Case studies demonstrating encapsulation and delivery efficiency of GenScript’s new LNP formulations in cell models, and mouse models via both IM and IV.

Free Download

Critical Consideration in Sourcing mRNA_Whitepaper

This whitepaper provides valuable insights into the essential factors that vaccine and therapy developers should consider when exploring mRNA solutions

Free Download

Advancements in mRNA manufacturing_eBook

Get GenScript’s free application eBook on therapeutics development with mRNA manufacturing solutions

Free Download

mRNA Therapy Modalities_Infographic

Learn about key structural features and functions of IVT mRNA and LNP, therapeutic modalities beyond prophylactic vaccines, mRNA candidates progressing through clinical trials.

Free Download

mRNA Parts_Infographic

Learn about the main structural components of mRNA, the functional roles of each part, what constitutes high-quality IVT mRNA and more.

Free Download

Circular RNA Beginner’s Guide_Infographic

Learn about key structural components of circRNA, the process by which circRNA is translated into protein, and more.

Free Download

Self-Amplifying RNA Beginner’s Guide_Infographic

Learn about key structural components of saRNA, the processes by which saRNA replicates and is translated into protein, and more.

Free Download